MCD99

mcd99Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ Balanced Salt Solution (HBSS). Cells were washed twice in PBS and lysated with 0.4% NP40When anti-mCD99 levels are hh, tumor cells are injected subcutaneously into the left flank of the mice (red arrow). Tumors are allowed to grow 3-4 weeks before the mice